How to Make Spawn Bags – Easiest Steps

Due to the increased demand for locally grown and organic foods, mushroom gardening has become more widespread recently. A crucial component of mushroom cultivation is the mushroom spawn bags, which contains the mycelium required to grow mushrooms. Mycelium, which consists of a web of threadlike cells, serves as the mushroom’s roots and is in charge of receiving nutrients from the substrate.

Numerous materials are used to create spawn bags, including polypropylene, which is preferred because of its toughness and resistance to sterilization.

These bags are filled with sterilized grains or sawdust that are inoculated with the desired strain of mushroom mycelium. Once the mycelium has fully colonized the substrate, the bag can be opened and transferred to a larger growing container or bed.

Spawn Bags

Spawn bags offer several advantages over other mushroom cultivation methods, including ease of use, low cost, and reduced risk of contamination. They also allow for year-round mushroom cultivation, regardless of outdoor weather conditions. Whether you’re a hobbyist or a professional grower, mushroom spawn bags are a great way to get started with mushroom cultivation.

Sterilization Tips For: Mushroom Spawn bags

  • Prepare a clean working area and gather all necessary equipment, including sterilized mushroom spawn bags, a pressure cooker, and a heat-resistant glove.
  • Fill the pressure cooker with water and place a rack or basket at the bottom to elevate the bags.
  • Make sure the bags of mushroom spawn are not in contact with one another or the pressure cooker’s sides when you carefully place them on the rack.
  • Secure the lid tightly on the pressure cooker and set it to the appropriate sterilization temperature and time recommended for your specific mushroom species.
  • Allow the pressure cooker to reach the desired temperature and maintain it for the specified time to ensure thorough sterilization.
  • After sterilization, carefully remove the bags from the pressure cooker using the heat-resistant glove and place them in a clean, sterile environment to cool down.
  • It is time to inoculate the bags with mushroom spawn when they have cooled.

Note: It’s essential to follow proper sterilization protocols and guidelines specific to your mushroom species to minimize contamination and ensure successful growth.

ALSO READ: How to Grow Oyster Mushrooms in Garden – Grower Guide

Inoculating Mushroom Spawn Bag

Inoculating Mushroom Spawn Bag

Inoculating a mushroom spawn bag is a key step in cultivating mushrooms at home. To remember, have the following in mind:

Sterilize the materials

Before inoculating the spawn bag, it’s important to sterilize all the materials you’ll be using to prevent contamination. This includes the spawn bag, the inoculation tool, and any other equipment.

Spawn preparation

The stuff that has mushroom mycelium in it is called spawn. You may buy pre-made spawn or build your own by sterilizing a substrate like grain or sawdust and utilizing it as your starting material. Inoculate the bag: Using a sterile inoculation tool, carefully inject the spawn into the bag through a small hole or injection port. you reduce the possibility of contamination, be sure you operate in an uncontaminated, sterile atmosphere.

Bag incubator

Seal the bag after inoculation and keep it in a warm, dark environment so the mycelium may develop. Be patient and keep an eye out for evidence of development on the bag frequently since this procedure might take many weeks.

Transfer to a fruiting container

Once the mycelium has fully colonized the spawn bag, you can transfer it to a fruiting container such as a terrarium or grow box to encourage fruiting and mushroom growth.

Colonization of the Mushroom Grain Spawn Bag

Colonization of a mushroom spawn bag typically involves the following steps:

  • Prepare the substrate mixture by combining sterilized grains or sawdust with mushroom spawn.
  • Fill the spawn bag with the substrate mixture, leaving some space at the top for the mycelium to grow.
  • Seal the bag to maintain a sterile environment, using an impulse sealer or another suitable method.
  • Incubate the bag at the optimal temperature and humidity for the specific mushroom species.
  • Give the mycelium enough time to completely colonize the substrate, which might take many weeks.
  • Check for white, web-like growth throughout the bag, indicating successful colonization.
  • Once colonization is complete, the bag is ready for the next stage, such as fruiting or transferring to a fruiting chamber.

Fruiting Your Spawn Bags

After inoculating your spawn bags with mushroom spores, it’s time to fruit them. First, make sure the bags are fully colonized with mycelium by checking for white, fluffy growth throughout the bag Create a few tiny holes in the bags so the mushrooms may grow through them. Maintain the bags in a humid area with excellent airflow and indirect lighting. You ought to notice mushrooms emerging from the holes in a few weeks. Harvest the mushrooms when they are fully grown but still firm, and enjoy!

Fruiting using the Bulk Casing Method

The Bulk Casing Method (BCM) is a popular technique used by mushroom growers to produce fruiting bodies of fungi. It involves mixing a colonized substrate, such as sawdust or straw, with a nutrient-rich casing layer, such as peat moss or vermiculite, in a large container. The mixture is then allowed to colonize further, creating a mycelial network throughout the container.

To encourage fruiting, the container is put in a humid environment when the mycelium has completely colonized the substrate and casing layer. This can be accomplished through the use of a misting system or by placing the container in a greenhouse or other controlled environment. Proper humidity levels are crucial for the development of healthy fruiting bodies.

It’s crucial to have the right levels of humidity, light, and temperature during the fruiting period. Most mushroom species prefer temperatures between 60 and 70 degrees Fahrenheit and require exposure to light in order to form fruiting bodies. Humidity levels should be kept at around 90% to promote proper growth and development.

Fruiting using the Bulk Casing Method

As the fruiting bodies develop, they should be harvested carefully to avoid damaging the mycelial network. To avoid contamination from other microbes throughout the harvesting process, it is crucial to maintain a clean and sterile atmosphere.

Fruiting in a Humidity Chamber

Fruiting in a humidity chamber is a great way to grow mushrooms indoors. Here are the steps to do it:

Prepare your substrate

Mix your mushroom spawn with your substrate, such as sawdust or straw.

Put the mixture into a container

Use a container with holes in the lid for air exchange.

Add water

Add enough water to the container to moisten the substrate. Be careful not to add too much.

Set up the humidity chamber

Put the container into the humidity chamber and set the humidity to 80-90%.

Monitor the temperature

The temperature should be around 70-75°F.

Wait for fruiting

The mushrooms will start to grow in 1-2 weeks.


Once the mushrooms are fully grown, harvest them and enjoy!

Fruiting in the Bag

Fruiting Bag

Fruiting in the bag is a simple and efficient method to grow mushrooms at home. The process involves inoculating sterilized substrate with mushroom spores and then allowing the mushrooms to fruit in a plastic bag. Here’s a step-by-step guide:

  • Sterilize the substrate by boiling or pressure cooking it for at least an hour.
  • Before spore-inoculating the substrate with mushrooms, let it cool. You can use a syringe or liquid culture for this.
  • Once the substrate is inoculated, seal it in a plastic bag and poke some small holes in the bag for ventilation.
  • Place the bag in a dark and humid area with a temperature range of 18-25°C.
  • After a few days, the substrate ought to begin to sprout white mycelium. This indicates that the mushrooms are expanding.
  • Once the mycelium has fully colonized the substrate, small mushrooms should start to grow from the holes in the bag.
  • When the mushrooms are mature and ready to eat, they should be harvested.

Fruiting in the bag is a great way to grow mushrooms for beginners as it is low maintenance and requires minimal equipment. Just make sure to follow proper sterilization techniques and maintain the ideal growing conditions for the best results.

Final Verdict

In conclusion, mushroom spawn bags are a convenient and efficient way to grow mushrooms at home or on a larger scale. They provide a sterile environment for the mycelium to grow and can be easily inoculated with spores or grain. With proper care and attention, mushroom spawn bags can yield bountiful harvests of delicious and nutritious mushrooms.

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